Recombinant DNA
The Southern blotting technique depends on

similarities between the sequences of probe protein and experimental protein
similarities between the sequences of probe RNA and experimental RNA
the molecular mass of proteins
similarities between the sequences of probe DNA and experimental DNA

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Recombinant DNA
Bacterial cells protect their own DNA from degradation by restriction endonucleases by

not producing any restriction endonucleases
deleting all recognition sites from the genome
methylating the DNA at the sites that the enzyme recognizes
having anti restriction endonucleases

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