Recombinant DNA
Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

expression vectors
BACS
YACS
all of these

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Recombinant DNA
Bacterial cells protect their own DNA from degradation by restriction endonucleases by

methylating the DNA at the sites that the enzyme recognizes
deleting all recognition sites from the genome
not producing any restriction endonucleases
having anti restriction endonucleases

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Recombinant DNA
The Southern blotting technique depends on

similarities between the sequences of probe RNA and experimental RNA
similarities between the sequences of probe DNA and experimental DNA
similarities between the sequences of probe protein and experimental protein
the molecular mass of proteins

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