Recombinant DNA
The Southern blotting technique depends on

similarities between the sequences of probe protein and experimental protein
the molecular mass of proteins
similarities between the sequences of probe RNA and experimental RNA
similarities between the sequences of probe DNA and experimental DNA

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Recombinant DNA
Vectors are

molecules that help in replication
molecules that degrade nucleic acids
molecules that protect host cells from invasion by foreign DNA
molecules that are able to covalently bond to and carry foreign DNA into cells

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