Recombinant DNA
Bacterial cells protect their own DNA from degradation by restriction endonucleases by

deleting all recognition sites from the genome
not producing any restriction endonucleases
methylating the DNA at the sites that the enzyme recognizes
having anti restriction endonucleases

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Recombinant DNA
The advantage of using DNA polymerases from thermophilic organisms in PCR is that

the DNA polymerases of these bacteria are much faster than those from other organisms
the DNA polymerases of these bacteria never make mistakes while replicating DNA
All of these
the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

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Recombinant DNA
The Southern blotting technique depends on

the molecular mass of proteins
similarities between the sequences of probe DNA and experimental DNA
similarities between the sequences of probe RNA and experimental RNA
similarities between the sequences of probe protein and experimental protein

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