Recombinant DNA
Bacterial cells protect their own DNA from degradation by restriction endonucleases by

having anti restriction endonucleases
methylating the DNA at the sites that the enzyme recognizes
deleting all recognition sites from the genome
not producing any restriction endonucleases

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Recombinant DNA
The Southern blotting technique depends on

the molecular mass of proteins
similarities between the sequences of probe RNA and experimental RNA
similarities between the sequences of probe DNA and experimental DNA
similarities between the sequences of probe protein and experimental protein

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