DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by helicase + ATP
are joined together by DNA ligase
result in Okazaki fragments on leading strand
are removed by Pol I

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DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
Tm = 69.3° + 0.41 (%)G+C -650/1
Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)

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DNA Structure and Replication
The B-DNA structure found in solution is a

right-handed double helix of parallel chains (∼10 bp/turn)
right-handed double helix of antiparallel chains (∼10 bp/turn)
left-handed zig-zag helix of antiparallel chains (10 bp/turn)
left-handed double helix of antiparallel chains (10 bp/turn)

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