DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

result in Okazaki fragments on leading strand
are removed by Pol I
are joined together by DNA ligase
are removed by helicase + ATP

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DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 69.3° + 0.41 (%)G+C -650/1
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

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