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DNA Structure and Replication

DNA Structure and Replication
DNA with a G-C content of 50% will melt at approximately

60°C
70 °C
90 °C
100 °C

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DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by Pol I
result in Okazaki fragments on leading strand
are removed by helicase + ATP
are joined together by DNA ligase

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DNA Structure and Replication
The melting of 40-45 bp at the oriC site of E. coli requires

DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
DNA+ ATP + HU + DNAb + DNAa
DNA + ATP + HU + DNAb
DNA + ATP + HU

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DNA Structure and Replication
What is the approximate size (in kb) of the E. coli genome?

6500 kilobase
4500 kilobase
3000 kilobase
5500 kilobase

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DNA Structure and Replication
A cesium chloride will separate DNA molecules by

density
absorption
resorption
adhesion

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DNA Structure and Replication
DNA helicase is used to

None of these
break a phosphodiester bond in DNA strand
unwind the double helix
interact the double helix closely

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