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DNA Structure and Replication

DNA Structure and Replication
DNA with a G-C content of 50% will melt at approximately

70 °C
60°C
100 °C
90 °C

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DNA Structure and Replication
In the study of one experiment it was found that the value of Tm for DNA is = 40° C. If the cell has 20% GC at the above Tm, then what will be value of 'Tm' if the GC% increases to 60%?

Remains same
Decreases
Increases
Can not be compared

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DNA Structure and Replication
Which will require a higher temperature to denature?

DNA with a high A-T content
DNA with a high G-C content
RNA with a high G-C content
RNA with a high A-T content

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DNA Structure and Replication
The DNA is negatively super coiled, complexes to histone,

H-NS
HSP-1
HU
All of these

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DNA Structure and Replication
What is the name given to the points at which a DNA helix is unwound and new strands develop?

Replication origins
Okazaki fragments
Replication forks
Leading strands

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DNA Structure and Replication
During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of

high temperature and Mg++
low temperature and Ca++
high temperature and Ca++
low temperature and Mg++

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