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DNA Structure and Replication

DNA Structure and Replication
DNA with a G-C content of 50% will melt at approximately

100 °C
90 °C
70 °C
60°C

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DNA Structure and Replication
The melting of 40-45 bp at the oriC site of E. coli requires

DNA + ATP + HU
DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
DNA + ATP + HU + DNAb
DNA+ ATP + HU + DNAb + DNAa

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DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by Pol I
result in Okazaki fragments on leading strand
are joined together by DNA ligase
are removed by helicase + ATP

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DNA Structure and Replication
The most common liquid volumes in molecular biology are measured in

ml
nl
μl
1

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DNA Structure and Replication
Enzymes, responsible for unraveling short segments of DNA is

helicase
primase
DNA polymerase
DNA ligase

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DNA Structure and Replication
In sperm heads, DNA is particularly highly condensed and the histones are replaced with small basic protein called

protamines
All of these
pyrimidines
purines

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