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DNA Structure and Replication

DNA Structure and Replication
DNA with a G-C content of 50% will melt at approximately

90 °C
70 °C
60°C
100 °C

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DNA Structure and Replication
Replication in E. coli is initiated by the generation of short RNA primers using

RNA polymerase
reverse transcriptase
both (a) and (b)
primase

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DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by Pol I
are removed by helicase + ATP
result in Okazaki fragments on leading strand
are joined together by DNA ligase

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DNA Structure and Replication
DNAs when charged, migrate in a gel towards the

positive pole
negative pole
will not migrate
none of these

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DNA Structure and Replication
The size of a linear DNA fragment in a gel can be estimated by

the sedimentation coefficient
the time it takes the gel to run
fluorescence in UV light
comparison with a molecular weight standard such as Hind III digested phage lambda

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DNA Structure and Replication
Which of the following in DNA replication and transcription are common?

synthesis in the 5'-3' direction
utilization the same enzyme
None of these
incorporation of deoxynucleotides

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