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DNA Structure and Replication

DNA Structure and Replication
DNA with a G-C content of 50% will melt at approximately

70 °C
60°C
100 °C
90 °C

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DNA Structure and Replication
The 5' and 3' numbers are related to the

the number of phosphates
carbon number in sugar
the base pair rule
length of the DNA strand

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DNA Structure and Replication
What is the range of melting point temperatures (Tm) for most DNA molecules?

80 to l00°C
50 to 60°C
60 to 80°C
70 to 90°C

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DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by helicase + ATP
result in Okazaki fragments on leading strand
are joined together by DNA ligase
are removed by Pol I

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DNA Structure and Replication
The role of primase is to

synthesize an RNA primer to begin the elongation process
proofread base pairing
cleave and unwinds short sections of DNA ahead of the replication fork
dismantle RNA primer

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DNA Structure and Replication
Automated DNA sequencing uses the chain termination method

but with an oligonucleotide primer labeled with a fluorescent dye
but with a polynucleotide primer labeled with a fluorescent dye
with no primer
either (b) or (c)

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MORE MCQ ON DNA Structure and Replication

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