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DNA Structure and Replication

DNA Structure and Replication
What is the approximate size (in kb) of the E. coli genome?

3000 kilobase
5500 kilobase
4500 kilobase
6500 kilobase

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DNA Structure and Replication
E.coli DNA polymerases II and III lack

5' → 3' endonuclease activity
5' → 3' exonuclease activity
partially 5' → 3' endonuclease activity
partially 5' → 3' exonuclease activity

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DNA Structure and Replication
The role of primase is to

synthesize an RNA primer to begin the elongation process
cleave and unwinds short sections of DNA ahead of the replication fork
proofread base pairing
dismantle RNA primer

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DNA Structure and Replication
During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of

high temperature and Mg++
low temperature and Mg++
low temperature and Ca++
high temperature and Ca++

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DNA Structure and Replication
The melting of 40-45 bp at the oriC site of E. coli requires

DNA + ATP + HU
DNA+ ATP + HU + DNAb + DNAa
DNA + ATP + HU + DNAb
DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme

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DNA Structure and Replication
The 5' and 3' numbers are related to the

carbon number in sugar
the number of phosphates
length of the DNA strand
the base pair rule

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