DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
Tm = 69.3° + 0.41 (%)G+C -650/1
Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)

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DNA Structure and Replication
In DNA, there are

five bases known as adenine, guanine, thymine, tryptophan and cytosine
four bases known as adenine, guanine, thymine and cytosine
three bases known as adenine, guanine and cytosine
only two bases known as adenine and cytosine

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DNA Structure and Replication
The B-DNA structure found in solution is a

right-handed double helix of parallel chains (∼10 bp/turn)
left-handed double helix of antiparallel chains (10 bp/turn)
left-handed zig-zag helix of antiparallel chains (10 bp/turn)
right-handed double helix of antiparallel chains (∼10 bp/turn)

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