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DNA Structure and Replication

DNA Structure and Replication
An RNA primer is synthesized by

DNA helicases
ligase
primase
DNA topoisomerase

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DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by helicase + ATP
are removed by Pol I
result in Okazaki fragments on leading strand
are joined together by DNA ligase

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DNA Structure and Replication
Automated DNA sequencing uses the chain termination method

either (b) or (c)
with no primer
but with an oligonucleotide primer labeled with a fluorescent dye
but with a polynucleotide primer labeled with a fluorescent dye

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DNA Structure and Replication
Which of the following is not a structural motif in DNA binding proteins?

bZIP
zinc finger
TFIID
helix-turn-helix

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DNA Structure and Replication
Klenow fragment without free nucleotides exhibits

exonuclease activity
no activity
endonuclease activity
nickase activity

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DNA Structure and Replication
One hypothesis of DNA replication suggested that the parental DNA molecules are broken into fragments. Both strands of DNA in each of the daughter molecules are made up of an assortment of parental and new DNA. This statement refers to which hypothesis?

Dispersive theory
Semi-conservative theory
Evolutionary theory
Conservative theory

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