DNA Structure and Replication
In DNA, there are

only two bases known as adenine and cytosine
four bases known as adenine, guanine, thymine and cytosine
three bases known as adenine, guanine and cytosine
five bases known as adenine, guanine, thymine, tryptophan and cytosine

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DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 69.3° + 0.41 (%)G+C -650/1
Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)

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