DNA Structure and Replication
DNA gyrase in E. coli

adds positive supercoils to chromosomal DNA
performs the same function as helicase in eukaryotes
can be inhibited with antibiotics
is required only at the oriC site

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DNA Structure and Replication
In DNA, nucleotides are covalently joined together by

3', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain
3', 4' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 3' phosphodiester bonds to form a repetitive sugar-phosphate chain

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