DNA Structure and Replication
DNA gyrase in E. coli

adds positive supercoils to chromosomal DNA
is required only at the oriC site
can be inhibited with antibiotics
performs the same function as helicase in eukaryotes

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DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
Tm = 69.3° + 0.41 (%)G+C -650/1
Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)

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