DNA Structure and Replication
DNA gyrase in E. coli

adds positive supercoils to chromosomal DNA
is required only at the oriC site
can be inhibited with antibiotics
performs the same function as helicase in eukaryotes

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DNA Structure and Replication
In DNA, nucleotides are covalently joined together by

2', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain
3', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain
3', 4' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 3' phosphodiester bonds to form a repetitive sugar-phosphate chain

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