DNA Structure and Replication
DNA gyrase in E. coli

can be inhibited with antibiotics
adds positive supercoils to chromosomal DNA
performs the same function as helicase in eukaryotes
is required only at the oriC site

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DNA Structure and Replication
The higher order structure of DNA shows symmetry, whereas the higher order structures of most proteins do not. Why isn't protein shape more regular like DNA?

DNA has one main function in cells whereas proteins have many.
All of these
The many different amino acid R groups on proteins confer many different shapes.
Some S amino acids cause proteins to bend; others cause protein to flatten.

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DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
Tm = 69.3° + 0.41 (%)G+C -650/1
Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)

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