DNA Structure and Replication
DNA gyrase in E. coli

adds positive supercoils to chromosomal DNA
is required only at the oriC site
can be inhibited with antibiotics
performs the same function as helicase in eukaryotes

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DNA Structure and Replication
The B-DNA structure found in solution is a

left-handed zig-zag helix of antiparallel chains (10 bp/turn)
left-handed double helix of antiparallel chains (10 bp/turn)
right-handed double helix of antiparallel chains (∼10 bp/turn)
right-handed double helix of parallel chains (∼10 bp/turn)

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