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DNA Structure and Replication

DNA Structure and Replication
Klenow fragment without free nucleotides exhibits

endonuclease activity
no activity
nickase activity
exonuclease activity

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DNA Structure and Replication
After one replication in 15N, how many bands should be observed in a cesium chloride gradient?

Two
One
A smear
Three

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DNA Structure and Replication
In a cesium chloride gradient, DNA labeled with 15N and centrifuged will form a band

all of these
below DNA containing 14N
with 14N-containing DNA
above DNA containing 14N

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DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by Pol I
are removed by helicase + ATP
result in Okazaki fragments on leading strand
are joined together by DNA ligase

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DNA Structure and Replication
Proteins involved in opening a replication bubble are

DNA helicases
single stranded binding proteins
DNA topoisomerase
ligase

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DNA Structure and Replication
The melting of 40-45 bp at the oriC site of E. coli requires

DNA + ATP + HU + DNAb
DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
DNA + ATP + HU
DNA+ ATP + HU + DNAb + DNAa

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