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DNA Structure and Replication

DNA Structure and Replication
Klenow fragment without free nucleotides exhibits

endonuclease activity
exonuclease activity
no activity
nickase activity

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DNA Structure and Replication
Which of the following is not a structural motif in DNA binding proteins?

helix-turn-helix
bZIP
zinc finger
TFIID

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DNA Structure and Replication
Which will require a higher temperature to denature?

RNA with a high A-T content
RNA with a high G-C content
DNA with a high G-C content
DNA with a high A-T content

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DNA Structure and Replication
E.coli DNA polymerases II and III lack

5' → 3' exonuclease activity
5' → 3' endonuclease activity
partially 5' → 3' endonuclease activity
partially 5' → 3' exonuclease activity

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DNA Structure and Replication
TM refers to the temperature at which

50% of a protein molecules are denatured
50% of a DNA molecule is denatured
membranes are 50% fluid
All of these

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DNA Structure and Replication
The size of a linear DNA fragment in a gel can be estimated by

comparison with a molecular weight standard such as Hind III digested phage lambda
the time it takes the gel to run
the sedimentation coefficient
fluorescence in UV light

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