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DNA Structure and Replication

DNA Structure and Replication
Klenow fragment without free nucleotides exhibits

no activity
exonuclease activity
nickase activity
endonuclease activity

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DNA Structure and Replication
The melting of 40-45 bp at the oriC site of E. coli requires

DNA + ATP + HU
DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
DNA + ATP + HU + DNAb
DNA+ ATP + HU + DNAb + DNAa

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DNA Structure and Replication
Which of the following in DNA replication and transcription are common?

synthesis in the 5'-3' direction
None of these
utilization the same enzyme
incorporation of deoxynucleotides

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DNA Structure and Replication
What is the name given to the points at which a DNA helix is unwound and new strands develop?

Replication origins
Leading strands
Replication forks
Okazaki fragments

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DNA Structure and Replication
TM refers to the temperature at which

50% of a DNA molecule is denatured
50% of a protein molecules are denatured
membranes are 50% fluid
All of these

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DNA Structure and Replication
What is the only common methylation in the DNA of eukaryotes?

Cytosine in CpG dinucleotides
Adenosine in GpA dinucleotides
None of these
Guanosine in ApGpA trinucleotides

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