DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 69.3° + 0.41 (%)G+C -650/1
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

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DNA Structure and Replication
Nucleosome is

an hexamer of three histones without H1 protein
an hexamer of three histones with H1 protein
an octamer of four histones with H1 protein
an octamer of four histones without H1 protein

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