DNA Structure and Replication
What does ligase do during replication of DNA?

Joins nicks in DNA strands
Makes copies of mRNA from DNA
Synthesizes the lagging strand
Digests mRNA when it is no longer needed

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DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 69.3° + 0.41 (%)G+C -650/1
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

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