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DNA Structure and Replication

DNA Structure and Replication
The most common liquid volumes in molecular biology are measured in

nl
ml
1
μl

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DNA Structure and Replication
TM refers to the temperature at which

membranes are 50% fluid
All of these
50% of a protein molecules are denatured
50% of a DNA molecule is denatured

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DNA Structure and Replication
DNA replication in eukaryotes occurs only in

S phase
G2 phase
G1 phase
M phase

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DNA Structure and Replication
What is the range of melting point temperatures (Tm) for most DNA molecules?

50 to 60°C
60 to 80°C
80 to l00°C
70 to 90°C

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DNA Structure and Replication
What is the main damaging effect of UV radiation on DNA?

Formation of thymine dimers
Dehydration
Single strand break
Depurination

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DNA Structure and Replication
The melting of 40-45 bp at the oriC site of E. coli requires

DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
DNA + ATP + HU + DNAb
DNA + ATP + HU
DNA+ ATP + HU + DNAb + DNAa

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