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DNA Structure and Replication

DNA Structure and Replication
In an agarose gel, DNA is moving from

anode to cathode
left to right
cathode to anode
A to B

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DNA Structure and Replication
Which will require a higher temperature to denature?

DNA with a high G-C content
RNA with a high G-C content
DNA with a high A-T content
RNA with a high A-T content

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DNA Structure and Replication
DNA gyrase in E. coli

can be inhibited with antibiotics
performs the same function as helicase in eukaryotes
is required only at the oriC site
adds positive supercoils to chromosomal DNA

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DNA Structure and Replication
During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of

high temperature and Ca++
high temperature and Mg++
low temperature and Mg++
low temperature and Ca++

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DNA Structure and Replication
What is the main damaging effect of UV radiation on DNA?

Dehydration
Formation of thymine dimers
Depurination
Single strand break

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DNA Structure and Replication
The 5' and 3' numbers are related to the

length of the DNA strand
the number of phosphates
the base pair rule
carbon number in sugar

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