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DNA Structure and Replication

DNA Structure and Replication
After one replication in 15N, how many bands should be observed in a cesium chloride gradient?

Two
One
Three
A smear

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DNA Structure and Replication
DNA can be sequenced by the

both (a) and (b)
chemical method
chain termination procedure
physical method

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DNA Structure and Replication
Okazaki fragments occur on the __________ and are bonded together by __________

Leading strand, polymerase
Lagging strand, ligase
tRNA, polymerase
mRNA, anticodons

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DNA Structure and Replication
The nicks or gaps between Okazaki fragments are eventually sealed by

gyrase
polymerase
RNA ligase
DNA ligase

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DNA Structure and Replication
In a cesium chloride gradient, DNA labeled with 15N and centrifuged will form a band

below DNA containing 14N
with 14N-containing DNA
all of these
above DNA containing 14N

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DNA Structure and Replication
The size of a linear DNA fragment in a gel can be estimated by

the sedimentation coefficient
fluorescence in UV light
the time it takes the gel to run
comparison with a molecular weight standard such as Hind III digested phage lambda

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