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DNA Structure and Replication

DNA Structure and Replication
A cesium chloride will separate DNA molecules by

absorption
density
adhesion
resorption

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DNA Structure and Replication
E.coli DNA polymerases II and III lack

partially 5' → 3' exonuclease activity
5' → 3' exonuclease activity
5' → 3' endonuclease activity
partially 5' → 3' endonuclease activity

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DNA Structure and Replication
DNA polymerases I also has

5'-3' exonuclease activity
both (a) and (b)
5'-3' endonuclease activity
3'-5'exonuclease activity

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DNA Structure and Replication
The size of a linear DNA fragment in a gel can be estimated by

fluorescence in UV light
the sedimentation coefficient
comparison with a molecular weight standard such as Hind III digested phage lambda
the time it takes the gel to run

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DNA Structure and Replication
DNA gyrase in E. coli

can be inhibited with antibiotics
is required only at the oriC site
performs the same function as helicase in eukaryotes
adds positive supercoils to chromosomal DNA

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DNA Structure and Replication
In the study of one experiment it was found that the value of Tm for DNA is = 40° C. If the cell has 20% GC at the above Tm, then what will be value of 'Tm' if the GC% increases to 60%?

Increases
Remains same
Decreases
Can not be compared

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