DNA Structure and Replication
TM refers to the temperature at which

50% of a protein molecules are denatured
All of these
membranes are 50% fluid
50% of a DNA molecule is denatured

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DNA Structure and Replication
The rapid appearance of HIV-1 strains that are resistant to AIDS drugs is due in part to which of the following property of its reverse transcriptase?

AZT (zidovudine) is too expensive to be taken in effective doses
It lacks a proofreading exonuclease
The RNase H domain of p66 causes error prone synthesis
It has low affinity for the correct dNTP's

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DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
Tm = 69.3° + 0.41 (%)G+C -650/1

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