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DNA Structure and Replication

DNA Structure and Replication
What is the range of melting point temperatures (Tm) for most DNA molecules?

80 to l00°C
70 to 90°C
60 to 80°C
50 to 60°C

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DNA Structure and Replication
During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of

high temperature and Ca++
low temperature and Ca++
low temperature and Mg++
high temperature and Mg++

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DNA Structure and Replication
Number of times the two DNA strands are interwined in circular DNA and is known as

linking number
none of these
twisting number
helix frequency

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DNA Structure and Replication
DNA polymerases I also has

3'-5'exonuclease activity
both (a) and (b)
5'-3' endonuclease activity
5'-3' exonuclease activity

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DNA Structure and Replication
What is the approximate size (in kb) of the E. coli genome?

4500 kilobase
6500 kilobase
3000 kilobase
5500 kilobase

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DNA Structure and Replication
Proteins which help to open the double helix for DNA synthesis are

ligase
single stranded binding proteins
DNA helicases
DNA topoisomerase

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