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DNA Structure and Replication

DNA Structure and Replication
What is the range of melting point temperatures (Tm) for most DNA molecules?

50 to 60°C
60 to 80°C
70 to 90°C
80 to l00°C

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DNA Structure and Replication
Replication in E. coli is initiated by the generation of short RNA primers using

reverse transcriptase
both (a) and (b)
RNA polymerase
primase

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DNA Structure and Replication
An important difference between eukaryotic and prokaryotic DNA replication is

more DNA polymerases are found in eukaryotes
RNA primers are not required in eukaryotes
eukaryotic DNA polymerases are faster
multiple origins of replication in eukaryotes

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DNA Structure and Replication
A cesium chloride will separate DNA molecules by

adhesion
density
resorption
absorption

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DNA Structure and Replication
The melting of 40-45 bp at the oriC site of E. coli requires

DNA + ATP + HU
DNA+ ATP + HU + DNAb + DNAa
DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
DNA + ATP + HU + DNAb

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DNA Structure and Replication
There is a single origin for DNA replication in bacteria. How many origins of replication are found in the much larger genomes of eukaryotes such as mammals?

One
Thousands and tens of thousands
About 100
none of these

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