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DNA Structure and Replication

DNA Structure and Replication
How does ethidium bromide interact with DNA?

It does not interact
It intercalates between bases
It binds to phosphate
It binds to adenine

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DNA Structure and Replication
E.coli DNA polymerases II and III lack

5' → 3' exonuclease activity
partially 5' → 3' endonuclease activity
partially 5' → 3' exonuclease activity
5' → 3' endonuclease activity

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DNA Structure and Replication
Proteins involved in opening a replication bubble are

DNA helicases
single stranded binding proteins
ligase
DNA topoisomerase

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DNA Structure and Replication
Automated DNA sequencing uses the chain termination method

either (b) or (c)
but with an oligonucleotide primer labeled with a fluorescent dye
with no primer
but with a polynucleotide primer labeled with a fluorescent dye

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DNA Structure and Replication
The DNA is negatively super coiled, complexes to histone,

All of these
H-NS
HSP-1
HU

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DNA Structure and Replication
After one replication in 15N, how many bands should be observed in a cesium chloride gradient?

Two
Three
A smear
One

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