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DNA Structure and Replication

DNA Structure and Replication
How does ethidium bromide interact with DNA?

It binds to phosphate
It binds to adenine
It does not interact
It intercalates between bases

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DNA Structure and Replication
Each replication bubbles consists of

2 replication forks
1 replication forks
3 replication forks
4 replication forks

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DNA Structure and Replication
The melting of 40-45 bp at the oriC site of E. coli requires

DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
DNA+ ATP + HU + DNAb + DNAa
DNA + ATP + HU
DNA + ATP + HU + DNAb

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DNA Structure and Replication
DNA gyrase in E. coli

performs the same function as helicase in eukaryotes
is required only at the oriC site
can be inhibited with antibiotics
adds positive supercoils to chromosomal DNA

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DNA Structure and Replication
Which will require a higher temperature to denature?

DNA with a high A-T content
RNA with a high G-C content
DNA with a high G-C content
RNA with a high A-T content

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DNA Structure and Replication
The nicks or gaps between Okazaki fragments are eventually sealed by

DNA ligase
polymerase
gyrase
RNA ligase

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