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DNA Structure and Replication

DNA Structure and Replication
The enzyme responsible for breaking the hydrogen bonds, and thus separating the DNA strands during DNA synthesis is

none of these
helicase
strandase
DNA polymerase

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DNA Structure and Replication
There is a single origin for DNA replication in bacteria. How many origins of replication are found in the much larger genomes of eukaryotes such as mammals?

Thousands and tens of thousands
One
none of these
About 100

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DNA Structure and Replication
What does ligase do during replication of DNA?

Synthesizes the lagging strand
Makes copies of mRNA from DNA
Digests mRNA when it is no longer needed
Joins nicks in DNA strands

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DNA Structure and Replication
DNA gyrase in E. coli

can be inhibited with antibiotics
performs the same function as helicase in eukaryotes
is required only at the oriC site
adds positive supercoils to chromosomal DNA

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DNA Structure and Replication
Which of the following is an active site on its single polypeptide chain for DNA Polymerase I?

3' → 5' exonuclease
All of these
5' → 3' exonuclease
5' → 3' polymerase

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DNA Structure and Replication
The most common liquid volumes in molecular biology are measured in

ml
nl
1
μl

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