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DNA Structure and Replication

DNA Structure and Replication
The enzyme responsible for breaking the hydrogen bonds, and thus separating the DNA strands during DNA synthesis is

strandase
DNA polymerase
helicase
none of these

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DNA Structure and Replication
DNAs when charged, migrate in a gel towards the

negative pole
positive pole
will not migrate
none of these

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DNA Structure and Replication
An important difference between eukaryotic and prokaryotic DNA replication is

more DNA polymerases are found in eukaryotes
multiple origins of replication in eukaryotes
RNA primers are not required in eukaryotes
eukaryotic DNA polymerases are faster

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DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by helicase + ATP
are removed by Pol I
are joined together by DNA ligase
result in Okazaki fragments on leading strand

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DNA Structure and Replication
The overall conclusion of the Hershey-Chase experiment was that

the ratio of Adenine to thymine was always the same
DNA was responsible for heredity
proteins and DNA were responsible for heredity
phage DNA was similar to bacterial DNA

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DNA Structure and Replication
DNA gyrase in E. coli

adds positive supercoils to chromosomal DNA
can be inhibited with antibiotics
performs the same function as helicase in eukaryotes
is required only at the oriC site

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