DNA Structure and Replication
In the study of one experiment it was found that the value of Tm for DNA is = 40° C. If the cell has 20% GC at the above Tm, then what will be value of 'Tm' if the GC% increases to 60%?

Increases
Can not be compared
Remains same
Decreases

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DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)
Tm = 69.3° + 0.41 (%)G+C -650/1

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