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DNA Structure and Replication

DNA Structure and Replication
The DNA is negatively super coiled, complexes to histone,

HSP-1
H-NS
HU
All of these

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DNA Structure and Replication
Automated DNA sequencing uses the chain termination method

but with an oligonucleotide primer labeled with a fluorescent dye
either (b) or (c)
but with a polynucleotide primer labeled with a fluorescent dye
with no primer

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DNA Structure and Replication
What is the approximate size (in kb) of the E. coli genome?

5500 kilobase
6500 kilobase
3000 kilobase
4500 kilobase

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DNA Structure and Replication
In DNA, guanine pairs with

Thymine
Uracil
Cytosine
Adenine

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DNA Structure and Replication
The role of primase is to

dismantle RNA primer
proofread base pairing
synthesize an RNA primer to begin the elongation process
cleave and unwinds short sections of DNA ahead of the replication fork

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DNA Structure and Replication
E.coli DNA polymerases II and III lack

partially 5' → 3' endonuclease activity
5' → 3' endonuclease activity
partially 5' → 3' exonuclease activity
5' → 3' exonuclease activity

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