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DNA Structure and Replication

DNA Structure and Replication
Okazaki fragments occur during

polymerase reaction
transformation
synthesis
replication

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DNA Structure and Replication
During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of

low temperature and Ca++
high temperature and Mg++
high temperature and Ca++
low temperature and Mg++

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DNA Structure and Replication
TM refers to the temperature at which

50% of a DNA molecule is denatured
50% of a protein molecules are denatured
All of these
membranes are 50% fluid

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DNA Structure and Replication
DNA can be sequenced by the

physical method
chain termination procedure
chemical method
both (a) and (b)

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DNA Structure and Replication
How does ethidium bromide interact with DNA?

It binds to phosphate
It does not interact
It intercalates between bases
It binds to adenine

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DNA Structure and Replication
What is the approximate size (in kb) of the E. coli genome?

5500 kilobase
4500 kilobase
6500 kilobase
3000 kilobase

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