DNA Structure and Replication
During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of

low temperature and Ca++
high temperature and Mg++
low temperature and Mg++
high temperature and Ca++

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DNA Structure and Replication
In DNA, nucleotides are covalently joined together by

3', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 3' phosphodiester bonds to form a repetitive sugar-phosphate chain
3', 4' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain

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