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DNA Structure and Replication

DNA Structure and Replication
DNAs when charged, migrate in a gel towards the

positive pole
negative pole
will not migrate
none of these

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DNA Structure and Replication
The nicks or gaps between Okazaki fragments are eventually sealed by

RNA ligase
polymerase
DNA ligase
gyrase

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DNA Structure and Replication
Replication in E. coli is initiated by the generation of short RNA primers using

both (a) and (b)
reverse transcriptase
primase
RNA polymerase

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DNA Structure and Replication
DNA gyrase in E. coli

performs the same function as helicase in eukaryotes
can be inhibited with antibiotics
adds positive supercoils to chromosomal DNA
is required only at the oriC site

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DNA Structure and Replication
Klenow fragment without free nucleotides exhibits

nickase activity
no activity
exonuclease activity
endonuclease activity

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DNA Structure and Replication
An important difference between eukaryotic and prokaryotic DNA replication is

RNA primers are not required in eukaryotes
multiple origins of replication in eukaryotes
eukaryotic DNA polymerases are faster
more DNA polymerases are found in eukaryotes

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