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DNA Structure and Replication

DNA Structure and Replication
DNAs when charged, migrate in a gel towards the

none of these
positive pole
will not migrate
negative pole

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DNA Structure and Replication
In a cesium chloride gradient, DNA labeled with 15N and centrifuged will form a band

above DNA containing 14N
all of these
with 14N-containing DNA
below DNA containing 14N

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DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by Pol I
are joined together by DNA ligase
result in Okazaki fragments on leading strand
are removed by helicase + ATP

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DNA Structure and Replication
What is the range of melting point temperatures (Tm) for most DNA molecules?

50 to 60°C
60 to 80°C
80 to l00°C
70 to 90°C

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DNA Structure and Replication
Reverse transcriptase produces

DNA from RNA
DNA from peptides
RNA from DNA
RNA from RNA

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DNA Structure and Replication
What bands will be observed in a cesium chloride gradient after two rounds of replication?

One medium and one heavy band
One light and one medium band
One light, one medium and one heavy band
One medium band

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