Monoclonal Antibodies
The EBV-hybridoma technique

All of these
facilitates the proliferation of antigen specific B cells
immortalizes the donor Bcells
gives much higher hybridization frequencies

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Monoclonal Antibodies
The approach (s), which is/are currently followed to produce human monoclonal antibodies, is/are known as

hybridization of 6-thioguanine-resistant human plasmacytoma with immune human lymphocytes
combination of EB Vand hybridoma techniques
transformation of antigen specific B lymphocytes (EBV)
all of these

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