Gel Electrophoresis Proteins are separated in an SDS-PAGE experiment on the basis of their positively charged side chains negatively charged side chains molecular weight different isoelectric points positively charged side chains negatively charged side chains molecular weight different isoelectric points ANSWER DOWNLOAD EXAMIANS APP
Gel Electrophoresis In a native PAGE, proteins are separated on the basis of net positive charges size net charge and size net negative charge net positive charge net positive charges size net charge and size net negative charge net positive charge ANSWER DOWNLOAD EXAMIANS APP
Gel Electrophoresis The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by isoelectric focusing combining information from (a)and (b) gel filtration chromatography SDS-PAGE electrophoresis isoelectric focusing combining information from (a)and (b) gel filtration chromatography SDS-PAGE electrophoresis ANSWER DOWNLOAD EXAMIANS APP
Gel Electrophoresis Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in both proteins migrate to the cathode both proteins migrate to the anode histones migrate to the anode and myoglobin migrates to the cathode histones migrate to the cathode and myoglobin migrates to the anode both proteins migrate to the cathode both proteins migrate to the anode histones migrate to the anode and myoglobin migrates to the cathode histones migrate to the cathode and myoglobin migrates to the anode ANSWER DOWNLOAD EXAMIANS APP
Gel Electrophoresis In an SDS-PAGE proteins have the same charge-to-mass ratio proteins are denatured by the SDS All of these smaller proteins migrate more rapidly through the gel proteins have the same charge-to-mass ratio proteins are denatured by the SDS All of these smaller proteins migrate more rapidly through the gel ANSWER DOWNLOAD EXAMIANS APP
Gel Electrophoresis In a gel filtration column both (a) and (b) large proteins elute first large proteins enter the beads more readily smaller proteins enter the beads more readily both (a) and (b) large proteins elute first large proteins enter the beads more readily smaller proteins enter the beads more readily ANSWER DOWNLOAD EXAMIANS APP
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