Gel Electrophoresis
In a gel filtration column

smaller proteins enter the beads more readily
large proteins elute first
large proteins enter the beads more readily
both (a) and (b)

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Gel Electrophoresis
In SDS-PAGE, the protein sample is first

None of these
treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis
treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

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Gel Electrophoresis
Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in

histones migrate to the anode and myoglobin migrates to the cathode
histones migrate to the cathode and myoglobin migrates to the anode
both proteins migrate to the anode
both proteins migrate to the cathode

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Gel Electrophoresis
In an SDS-PAGE

proteins are denatured by the SDS
All of these
smaller proteins migrate more rapidly through the gel
proteins have the same charge-to-mass ratio

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