Gel Electrophoresis
In isoelectric focusing, proteins are separated on the basis of their

size
relative content of positively and negatively charged residue
relative content of negatively charged residue only
relative content of positively charged residue only

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Gel Electrophoresis
In an SDS-PAGE

proteins are denatured by the SDS
smaller proteins migrate more rapidly through the gel
All of these
proteins have the same charge-to-mass ratio

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