Gel Electrophoresis
In SDS-PAGE, the protein sample is first

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis
treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.
None of these

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