Gel Electrophoresis
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by

gel filtration chromatography
combining information from (a)and (b)
SDS-PAGE electrophoresis
isoelectric focusing

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Gel Electrophoresis
In an SDS-PAGE

smaller proteins migrate more rapidly through the gel
proteins have the same charge-to-mass ratio
All of these
proteins are denatured by the SDS

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