Gel Electrophoresis
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by

gel filtration chromatography
isoelectric focusing
combining information from (a)and (b)
SDS-PAGE electrophoresis

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Gel Electrophoresis
In an SDS-PAGE

proteins have the same charge-to-mass ratio
All of these
smaller proteins migrate more rapidly through the gel
proteins are denatured by the SDS

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Gel Electrophoresis
Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in

both proteins migrate to the anode
histones migrate to the cathode and myoglobin migrates to the anode
both proteins migrate to the cathode
histones migrate to the anode and myoglobin migrates to the cathode

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