Gel Electrophoresis
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by

combining information from (a)and (b)
isoelectric focusing
gel filtration chromatography
SDS-PAGE electrophoresis

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Gel Electrophoresis
In an SDS-PAGE

proteins have the same charge-to-mass ratio
All of these
proteins are denatured by the SDS
smaller proteins migrate more rapidly through the gel

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Gel Electrophoresis
In SDS-PAGE, the protein sample is first

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis
treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis
None of these
fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

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