Gel Electrophoresis
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by

SDS-PAGE electrophoresis
isoelectric focusing
gel filtration chromatography
combining information from (a)and (b)

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Gel Electrophoresis
In SDS-PAGE, the protein sample is first

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis
fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.
None of these
treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

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