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GEL ELECTROPHORESIS

Gel Electrophoresis
In a gel filtration column

smaller proteins enter the beads more readily
large proteins enter the beads more readily
both (a) and (b)
large proteins elute first

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Gel Electrophoresis
In a native PAGE, proteins are separated on the basis of

net positive charges size
net positive charge
net negative charge
net charge and size

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Gel Electrophoresis
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by

SDS-PAGE electrophoresis
combining information from (a)and (b)
gel filtration chromatography
isoelectric focusing

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Gel Electrophoresis
Proteins are separated in an SDS-PAGE experiment on the basis of their

molecular weight
different isoelectric points
positively charged side chains
negatively charged side chains

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