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GEL ELECTROPHORESIS

Gel Electrophoresis
In a gel filtration column

large proteins enter the beads more readily
both (a) and (b)
large proteins elute first
smaller proteins enter the beads more readily

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Gel Electrophoresis
In a native PAGE, proteins are separated on the basis of

net negative charge
net positive charge
net charge and size
net positive charges size

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Gel Electrophoresis
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by

combining information from (a)and (b)
isoelectric focusing
SDS-PAGE electrophoresis
gel filtration chromatography

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Gel Electrophoresis
Proteins are separated in an SDS-PAGE experiment on the basis of their

different isoelectric points
negatively charged side chains
molecular weight
positively charged side chains

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