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DNA Structure and Replication

DNA Structure and Replication
Enzyme, responsible for proofreading base pairing is

DNA polymerase
DNA ligase
Primase
Telomerase

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DNA Structure and Replication
The melting of 40-45 bp at the oriC site of E. coli requires

DNA+ ATP + HU + DNAb + DNAa
DNA + ATP + HU
DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme
DNA + ATP + HU + DNAb

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DNA Structure and Replication
Klenow fragment without free nucleotides exhibits

endonuclease activity
no activity
exonuclease activity
nickase activity

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DNA Structure and Replication
What does ligase do during replication of DNA?

Digests mRNA when it is no longer needed
Synthesizes the lagging strand
Makes copies of mRNA from DNA
Joins nicks in DNA strands

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DNA Structure and Replication
Which of the following enzyme adds complementary bases during replication?

Polymerase
Replicase
Synthesase
Helicase

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DNA Structure and Replication
E.coli DNA polymerases II and III lack

partially 5' → 3' endonuclease activity
5' → 3' endonuclease activity
5' → 3' exonuclease activity
partially 5' → 3' exonuclease activity

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