DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
Tm = 69.3° + 0.41 (%)G+C -650/1
Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

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DNA Structure and Replication
Taylor, Woods and Hughes labeled Vicia DNA by allowing new DNA synthesis in the presence of radioactive thymine. After DNA replication (S phase of the cell cycle), it was observed that

both chromatids of a chromosome were labeled
only one chromatid of a chromosome was labeled
both (a) and (b)
neither chromatid was labeled

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