DNA Structure and Replication Which of the following is used to determine the appropriate stringency (Wallace rule)? Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair) Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair) Tm = 69.3° + 0.41 (%)G+C -650/1 Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair) Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair) Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair) Tm = 69.3° + 0.41 (%)G+C -650/1 Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair) ANSWER DOWNLOAD EXAMIANS APP
DNA Structure and Replication Proteins involved in opening a replication bubble are DNA helicases ligase single stranded binding proteins DNA topoisomerase DNA helicases ligase single stranded binding proteins DNA topoisomerase ANSWER DOWNLOAD EXAMIANS APP
DNA Structure and Replication The RNA primers used to initiate replication in E. coli result in Okazaki fragments on leading strand are removed by Pol I are removed by helicase + ATP are joined together by DNA ligase result in Okazaki fragments on leading strand are removed by Pol I are removed by helicase + ATP are joined together by DNA ligase ANSWER DOWNLOAD EXAMIANS APP
DNA Structure and Replication If one cell has AT contents 40%, what will be the percentage of Guanine residue? 30% 15% Guanine residue can not be calculated 60% 30% 15% Guanine residue can not be calculated 60% ANSWER DOWNLOAD EXAMIANS APP
DNA Structure and Replication The size of a linear DNA fragment in a gel can be estimated by fluorescence in UV light the time it takes the gel to run comparison with a molecular weight standard such as Hind III digested phage lambda the sedimentation coefficient fluorescence in UV light the time it takes the gel to run comparison with a molecular weight standard such as Hind III digested phage lambda the sedimentation coefficient ANSWER DOWNLOAD EXAMIANS APP
DNA Structure and Replication What is the range of melting point temperatures (Tm) for most DNA molecules? 60 to 80°C 80 to l00°C 70 to 90°C 50 to 60°C 60 to 80°C 80 to l00°C 70 to 90°C 50 to 60°C ANSWER DOWNLOAD EXAMIANS APP
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