DNA Structure and Replication
Which of the following is used to determine the appropriate stringency (Wallace rule)?

Tm = 69.3° + 0.41 (%)G+C -650/1
Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair)
Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair)
Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair)

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DNA Structure and Replication
Nucleosome is

an hexamer of three histones without H1 protein
an octamer of four histones without H1 protein
an hexamer of three histones with H1 protein
an octamer of four histones with H1 protein

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DNA Structure and Replication
In DNA, nucleotides are covalently joined together by

3', 4' phosphodiester bonds to form a repetitive sugar-phosphate chain
3', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 3' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain

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DNA Structure and Replication
The rapid appearance of HIV-1 strains that are resistant to AIDS drugs is due in part to which of the following property of its reverse transcriptase?

It lacks a proofreading exonuclease
AZT (zidovudine) is too expensive to be taken in effective doses
It has low affinity for the correct dNTP's
The RNase H domain of p66 causes error prone synthesis

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