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DNA Structure and Replication

DNA Structure and Replication
Proteins involved in opening a replication bubble are

DNA helicases
DNA topoisomerase
ligase
single stranded binding proteins

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DNA Structure and Replication
The role of primase is to

cleave and unwinds short sections of DNA ahead of the replication fork
synthesize an RNA primer to begin the elongation process
dismantle RNA primer
proofread base pairing

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DNA Structure and Replication
The size of a linear DNA fragment in a gel can be estimated by

the time it takes the gel to run
comparison with a molecular weight standard such as Hind III digested phage lambda
fluorescence in UV light
the sedimentation coefficient

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DNA Structure and Replication
The nicks or gaps between Okazaki fragments are eventually sealed by

gyrase
polymerase
RNA ligase
DNA ligase

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DNA Structure and Replication
What is the name given to the points at which a DNA helix is unwound and new strands develop?

Replication origins
Leading strands
Okazaki fragments
Replication forks

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DNA Structure and Replication
One hypothesis of DNA replication suggested that the parental DNA molecules are broken into fragments. Both strands of DNA in each of the daughter molecules are made up of an assortment of parental and new DNA. This statement refers to which hypothesis?

Dispersive theory
Conservative theory
Evolutionary theory
Semi-conservative theory

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