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DNA Structure and Replication

DNA Structure and Replication
Proteins involved in opening a replication bubble are

DNA helicases
ligase
DNA topoisomerase
single stranded binding proteins

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DNA Structure and Replication
What is the main damaging effect of UV radiation on DNA?

Single strand break
Formation of thymine dimers
Dehydration
Depurination

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DNA Structure and Replication
DNA gyrase in E. coli

is required only at the oriC site
performs the same function as helicase in eukaryotes
adds positive supercoils to chromosomal DNA
can be inhibited with antibiotics

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DNA Structure and Replication
Automated DNA sequencing uses the chain termination method

but with a polynucleotide primer labeled with a fluorescent dye
either (b) or (c)
but with an oligonucleotide primer labeled with a fluorescent dye
with no primer

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DNA Structure and Replication
A cesium chloride will separate DNA molecules by

resorption
density
absorption
adhesion

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DNA Structure and Replication
Which of the following enzyme adds complementary bases during replication?

Replicase
Helicase
Polymerase
Synthesase

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