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DNA Structure and Replication

DNA Structure and Replication
When DNA polymerase is in contact with guanine in the parental strand, what does it add to the growing daughter strand?

Uracil
Phosphate
Cytosine
Guanine

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DNA Structure and Replication
In the study of one experiment it was found that the value of Tm for DNA is = 40° C. If the cell has 20% GC at the above Tm, then what will be value of 'Tm' if the GC% increases to 60%?

Decreases
Can not be compared
Increases
Remains same

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DNA Structure and Replication
Which DNA polymerase removes RNA primers in DNA synthesis?

Polymerase I
Polymerase II
none of these
Polymerase III

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DNA Structure and Replication
DNA gyrase in E. coli

is required only at the oriC site
can be inhibited with antibiotics
adds positive supercoils to chromosomal DNA
performs the same function as helicase in eukaryotes

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DNA Structure and Replication
DNA can be sequenced by the

physical method
chain termination procedure
both (a) and (b)
chemical method

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DNA Structure and Replication
What is the range of melting point temperatures (Tm) for most DNA molecules?

70 to 90°C
80 to l00°C
50 to 60°C
60 to 80°C

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