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DNA Structure and Replication

DNA Structure and Replication
Enzymes, responsible for unraveling short segments of DNA is

DNA ligase
helicase
DNA polymerase
primase

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DNA Structure and Replication
In a cesium chloride gradient, DNA labeled with 15N and centrifuged will form a band

below DNA containing 14N
with 14N-containing DNA
above DNA containing 14N
all of these

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DNA Structure and Replication
One hypothesis of DNA replication suggested that the parental DNA molecules are broken into fragments. Both strands of DNA in each of the daughter molecules are made up of an assortment of parental and new DNA. This statement refers to which hypothesis?

Semi-conservative theory
Conservative theory
Evolutionary theory
Dispersive theory

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DNA Structure and Replication
What is the range of melting point temperatures (Tm) for most DNA molecules?

80 to l00°C
60 to 80°C
70 to 90°C
50 to 60°C

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DNA Structure and Replication
During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of

low temperature and Ca++
low temperature and Mg++
high temperature and Ca++
high temperature and Mg++

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DNA Structure and Replication
An important difference between eukaryotic and prokaryotic DNA replication is

eukaryotic DNA polymerases are faster
more DNA polymerases are found in eukaryotes
RNA primers are not required in eukaryotes
multiple origins of replication in eukaryotes

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