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DNA Structure and Replication

DNA Structure and Replication
Enzymes, responsible for unraveling short segments of DNA is

DNA polymerase
primase
DNA ligase
helicase

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DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by helicase + ATP
are joined together by DNA ligase
are removed by Pol I
result in Okazaki fragments on leading strand

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DNA Structure and Replication
The replication of a linear DNA molecule in a eukaryotic chromosome creates a problem which is reported to be sorted out by

using enzyme called telomerase
using RNA as a template
None of these
shortening of the double stranded replicated portion

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DNA Structure and Replication
E.coli DNA polymerases II and III lack

5' → 3' exonuclease activity
5' → 3' endonuclease activity
partially 5' → 3' endonuclease activity
partially 5' → 3' exonuclease activity

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DNA Structure and Replication
Replication in E. coli is initiated by the generation of short RNA primers using

RNA polymerase
primase
reverse transcriptase
both (a) and (b)

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DNA Structure and Replication
What bands will be observed in a cesium chloride gradient after two rounds of replication?

One light and one medium band
One medium and one heavy band
One light, one medium and one heavy band
One medium band

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