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DNA Structure and Replication

DNA Structure and Replication
Enzymes, responsible for unraveling short segments of DNA is

helicase
DNA polymerase
primase
DNA ligase

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DNA Structure and Replication
DNA replication takes place in which direction?

5 'to 3'
Randomly
Vary from organism to organism
3' to 5'

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DNA Structure and Replication
What is the range of melting point temperatures (Tm) for most DNA molecules?

80 to l00°C
50 to 60°C
70 to 90°C
60 to 80°C

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DNA Structure and Replication
Klenow fragment without free nucleotides exhibits

exonuclease activity
no activity
endonuclease activity
nickase activity

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DNA Structure and Replication
The 5' and 3' numbers are related to the

the number of phosphates
the base pair rule
length of the DNA strand
carbon number in sugar

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DNA Structure and Replication
DNA can be sequenced by the

physical method
both (a) and (b)
chain termination procedure
chemical method

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