DNA Sequencing, Mutation and Repair
Differentiation of four different bases in automated sequencing systems is that each base has

different radioactive tag
distinctive fluorescent tag
cytosine at start
unique antibody bound to it

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DNA Sequencing, Mutation and Repair
The DNA sequences of rRNA genes can be used to

assess relatedness of eukaryotes but not prokaryotes
obtain a tentative identification of a new microorganism
predict whether a bacteria will be gram-positive or gram-negative
predict the pulsed field gel pattern of a microorganism

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DNA Sequencing, Mutation and Repair
The whole-genome shotgun sequencing approach depends primarily on

rapidly sequencing thousands of small randomly cloned fragments
sequencing the bacterial chromosome while it is still intact
All of these
methodical sequencing a few large cloned fragments of DNA

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