DNA Sequencing, Mutation and Repair
Differentiation of four different bases in automated sequencing systems is that each base has

distinctive fluorescent tag
unique antibody bound to it
cytosine at start
different radioactive tag

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DNA Sequencing, Mutation and Repair
The whole-genome shotgun sequencing approach depends primarily on

All of these
sequencing the bacterial chromosome while it is still intact
methodical sequencing a few large cloned fragments of DNA
rapidly sequencing thousands of small randomly cloned fragments

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