DNA Sequencing, Mutation and Repair
The whole-genome shotgun sequencing approach depends primarily on

All of these
sequencing the bacterial chromosome while it is still intact
methodical sequencing a few large cloned fragments of DNA
rapidly sequencing thousands of small randomly cloned fragments

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DNA Sequencing, Mutation and Repair
The DNA sequences of rRNA genes can be used to

predict whether a bacteria will be gram-positive or gram-negative
assess relatedness of eukaryotes but not prokaryotes
predict the pulsed field gel pattern of a microorganism
obtain a tentative identification of a new microorganism

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