DNA Sequencing, Mutation and Repair
A nonsense mutation may result into

a large deletion within the reading frame of a gene
modification of mRNA
a premature termination of the synthesis of a polypeptide
an abnormal elongation of a polypeptide

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DNA Sequencing, Mutation and Repair
The DNA sequences of rRNA genes can be used to

predict whether a bacteria will be gram-positive or gram-negative
obtain a tentative identification of a new microorganism
predict the pulsed field gel pattern of a microorganism
assess relatedness of eukaryotes but not prokaryotes

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DNA Sequencing, Mutation and Repair
The whole-genome shotgun sequencing approach depends primarily on

sequencing the bacterial chromosome while it is still intact
rapidly sequencing thousands of small randomly cloned fragments
All of these
methodical sequencing a few large cloned fragments of DNA

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