DNA Sequencing, Mutation and Repair
A nonsense mutation may result into

an abnormal elongation of a polypeptide
modification of mRNA
a large deletion within the reading frame of a gene
a premature termination of the synthesis of a polypeptide

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DNA Sequencing, Mutation and Repair
The whole-genome shotgun sequencing approach depends primarily on

rapidly sequencing thousands of small randomly cloned fragments
methodical sequencing a few large cloned fragments of DNA
All of these
sequencing the bacterial chromosome while it is still intact

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