DNA Sequencing, Mutation and Repair
A nonsense mutation introduced into the DNA sequence of a gene may

cause premature termination of the mRNA
have no effect on the transcript or protein made
cause a shift in reading frame
shorten the length of the protein encoded by the gene

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DNA Sequencing, Mutation and Repair
The DNA sequences of rRNA genes can be used to

assess relatedness of eukaryotes but not prokaryotes
predict whether a bacteria will be gram-positive or gram-negative
predict the pulsed field gel pattern of a microorganism
obtain a tentative identification of a new microorganism

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DNA Sequencing, Mutation and Repair
The whole-genome shotgun sequencing approach depends primarily on

sequencing the bacterial chromosome while it is still intact
methodical sequencing a few large cloned fragments of DNA
rapidly sequencing thousands of small randomly cloned fragments
All of these

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DNA Sequencing, Mutation and Repair
Due to which of the following DNA repair mechanisms, one is able to distinguish newly synthesized DNA strands from older one?

New strands do not contain cytosine bases
New strands are methylated while old strands are not
New strands are lower in molecular size
Old strands are methylated while new strands are not

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