DNA Sequencing, Mutation and Repair
A nonsense mutation introduced into the DNA sequence of a gene may

shorten the length of the protein encoded by the gene
cause premature termination of the mRNA
cause a shift in reading frame
have no effect on the transcript or protein made

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DNA Sequencing, Mutation and Repair
The whole-genome shotgun sequencing approach depends primarily on

rapidly sequencing thousands of small randomly cloned fragments
All of these
sequencing the bacterial chromosome while it is still intact
methodical sequencing a few large cloned fragments of DNA

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