Vectors Uses for Animal Cell Culture
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

coding for virus coat protein
important for packaging of viral DNA into viral head
not necessary for viral replication
transcriptional regulators and are essential for viral replication

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Vectors Uses for Animal Cell Culture
In case of Baculoviruses, the foreign gene

is inserted randomly in viral genome
is inserted downstream of polyhedrin promoter
is inserted between the polyhedrin promoter and downstream sequences
is inserted downstream of viral origin of replication

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Vectors Uses for Animal Cell Culture
Retroviruses have advantage for being used as vector for animal cells because

infection does not lead to cell death, infected cells produce virus over an indefinite period
All of these
viral gene expression is driven by strong promoters
they cover a wide host range

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