Recombinant DNA
Vectors are

molecules that degrade nucleic acids
molecules that help in replication
molecules that protect host cells from invasion by foreign DNA
molecules that are able to covalently bond to and carry foreign DNA into cells

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Recombinant DNA
Bacterial cells protect their own DNA from degradation by restriction endonucleases by

deleting all recognition sites from the genome
not producing any restriction endonucleases
methylating the DNA at the sites that the enzyme recognizes
having anti restriction endonucleases

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Recombinant DNA
The Southern blotting technique depends on

similarities between the sequences of probe DNA and experimental DNA
similarities between the sequences of probe protein and experimental protein
similarities between the sequences of probe RNA and experimental RNA
the molecular mass of proteins

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