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Recombinant DNA

Recombinant DNA
Which of the following is obtained using processed mRNA molecules as a template?

tDNA
mDNA
cDNA
rDNA

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Recombinant DNA
For gene probes to be useful they must

None of these
both (a) and (b)
be labeled in some manner to allow detection
be large enough to contain gene-specific sequences

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Recombinant DNA
Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

DNA polymerase
Restriction endonuclease
DNA helicase
DNA ligase

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Recombinant DNA
What is the normal role of restriction endonucleases in bacterial cells?

To produce RNA primers for replication
All of these
To degrade invading phage DNA
To degrade the bacterial chromosome into small pieces during replication

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Recombinant DNA
The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

microbiology
genetic engineering
protein engineering
population genetics

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Recombinant DNA
Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

expression vectors
BACS
YACS
all of these

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