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Cell Cultures and Characteristics

Cell Cultures and Characteristics
Which of the following techniques may be performed quantitatively to determine the number of bacteria of a particular type?

Both (a) and (b)
Pour plate
Spread plate
Streak plate technique

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Cell Cultures and Characteristics
Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually inhibited.

sugars
low levels of nitrogen
bile salts
low concentration of various dyes

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Cell Cultures and Characteristics
In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

-30 to -48°C
-60 to -78°C
-48 to -58°C
-20 to -30°C

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Cell Cultures and Characteristics
To select cholera-causing bacterium, Vibrio cholerae, the pH of the medium is maintained at a pH of

8.90
3.5
8.5
6.79

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Cell Cultures and Characteristics
Which of the following is used for the proper maintenance and preservation of pure cultures?

Periodic transfer to fresh media
Preservation by lyophilization
All of these
Preservation by overlaying cultures with mineral oil

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Cell Cultures and Characteristics
Which of the following bacteria dies quickly after drying?

Mycobacterium tuberculosis
Pseudomonas aeruginosa
Treponema pallidium
Staphylococcus aureus

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