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Cell Cultures and Characteristics

Cell Cultures and Characteristics
In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

-30 to -48°C
-20 to -30°C
-60 to -78°C
-48 to -58°C

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Cell Cultures and Characteristics
Cell size and cell number can be measured using

hemocytometer
coulter counter
none of these
petroff-hausser counting chamber

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Cell Cultures and Characteristics
Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually inhibited.

low levels of nitrogen
sugars
low concentration of various dyes
bile salts

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Cell Cultures and Characteristics
Selective media facilitate growth of only one kind of organism. Saboraud medium is used to selectively isolate

gram positive bacteria
coliform bacteria
acid fast organisms
yeasts

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Cell Cultures and Characteristics
When a substance is added to a liquid medium which inhibits the growth of unwanted bacteria and favors the growth of wanted bacteria, it is known as

enrichment medium
differential medium
enriched medium
selective medium

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Cell Cultures and Characteristics
Viable plate count can be obtained using

Spread plate method
Hemocytometer
Pour plate method
both (a) and (b)

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