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Cell Cultures and Characteristics

Cell Cultures and Characteristics
In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

-20 to -30°C
-30 to -48°C
-60 to -78°C
-48 to -58°C

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Cell Cultures and Characteristics
Plate counts are often reported as

Both (a) and (b)
cfu/ml
cfu
thousands/ml

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Cell Cultures and Characteristics
Which of the following methods is most likely to be quantitative?

Gram staining
Wet mount
Dilution and plating
None of these

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Cell Cultures and Characteristics
Which of the following is used for the proper maintenance and preservation of pure cultures?

Preservation by overlaying cultures with mineral oil
All of these
Preservation by lyophilization
Periodic transfer to fresh media

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Cell Cultures and Characteristics
The solidifying agent commonly used in preparation of media is/are

agar
both (a) and (b)
none of these
silica gel

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Cell Cultures and Characteristics
Which of the following hint(s) suggest/s that a given specimen is likely to contain anaerobic bacteria?

Foul odour
Gas in specimen
All of these
Presence of sulphur granules

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