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Cell Cultures and Characteristics

Cell Cultures and Characteristics
In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

-30 to -48°C
-20 to -30°C
-60 to -78°C
-48 to -58°C

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Cell Cultures and Characteristics
Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually inhibited.

sugars
bile salts
low concentration of various dyes
low levels of nitrogen

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Cell Cultures and Characteristics
The concentration of agar to obtain semi solid media is

>10%but<20%
0.5% or less
1.5-20%
>10%

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Cell Cultures and Characteristics
Which of the following techniques may be performed quantitatively to determine the number of bacteria of a particular type?

Spread plate
Both (a) and (b)
Pour plate
Streak plate technique

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Cell Cultures and Characteristics
Agar is used for solidifying culture media because

it does not affect by the growth of bacteria
All of these
it does not add to the nutritive properties of the medium
the melting and solidifying points of agar solution are not the same

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Cell Cultures and Characteristics
Which of the following is used for the proper maintenance and preservation of pure cultures?

Periodic transfer to fresh media
Preservation by lyophilization
Preservation by overlaying cultures with mineral oil
All of these

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