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Cell Cultures and Characteristics

Cell Cultures and Characteristics
In the process of freeze drying, a dense cell suspension is placed in small vials and is frozen at

-30 to -48°C
-60 to -78°C
-20 to -30°C
-48 to -58°C

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Cell Cultures and Characteristics
Intestinal bacteria can grow in the presence of __________ whereas nonintestinal bacteria are usually inhibited.

sugars
low concentration of various dyes
low levels of nitrogen
bile salts

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Cell Cultures and Characteristics
The medium which allows the growth of more than one microorganisms of interest but with morphologically distinguishable colonies is known as

enrichment medium
selective medium
none of these
differential medium

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Cell Cultures and Characteristics
When a substance is added to a solid medium which inhibits the growth of unwanted bacteria but permits the growth of wanted bacteria, it is known as

enriched medium
selective medium
enrichment medium
differential medium

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Cell Cultures and Characteristics
Viable plate count can be obtained using

Pour plate method
Spread plate method
both (a) and (b)
Hemocytometer

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Cell Cultures and Characteristics
Selective media facilitate growth of only one kind of organism. Saboraud medium is used to selectively isolate

coliform bacteria
yeasts
gram positive bacteria
acid fast organisms

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