Microbiology of Soils
The transformation of nitrates to gaseous nitrogen is accomplished by microorganisms in a series of biochemical reactions. The process is known as

nitrification
ammonification
denitrification
nitrogen fixation

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Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

measuring15N2 by mass spectrometer
All of these
demonstrating growth in a nitrogen free medium
cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen

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