Microbiology of Soils
Nitrifying bacteria can not be isolated directly by the usual techniques employed to isolate hetrotrophic bacteria. The reasons may be due to

medium growth
slow growth
none of these
fast growth

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Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen
demonstrating growth in a nitrogen free medium
All of these
measuring15N2 by mass spectrometer

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Microbiology of Soils
Denitrification is

both (a) and (b)
reduction of nitrate to organic nitrogen compounds
reduction of nitrate (NO3-) to nitrogen gas
changing of atmospheric nitrogen (N2) to nitrogen compounds

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