Microbiology of Soils
Nitrifying bacteria can not be isolated directly by the usual techniques employed to isolate hetrotrophic bacteria. The reasons may be due to

none of these
slow growth
medium growth
fast growth

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Microbiology of Soils
Nitrogen fixation

changes the free nitrogen (N2) to a form usable by plants
fix the free nitrogen (N2) by which it should not be usable by plants
especially changes nitrogen compounds, mostly amines such as NH2
both (a) and (b)

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