Microbiology of Soils
Nitrifying bacteria can not be isolated directly by the usual techniques employed to isolate hetrotrophic bacteria. The reasons may be due to

none of these
fast growth
slow growth
medium growth

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Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

measuring15N2 by mass spectrometer
cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen
demonstrating growth in a nitrogen free medium
All of these

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