Microbiology of Soils
Nitrifying bacteria can not be isolated directly by the usual techniques employed to isolate hetrotrophic bacteria. The reasons may be due to

fast growth
medium growth
slow growth
none of these

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Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

All of these
cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen
measuring15N2 by mass spectrometer
demonstrating growth in a nitrogen free medium

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